Peptide synthesis technology

solid-phase synthesis

in 1963, the Merrifield first proposed method of solid-phase peptide synthesis (SPPS), due to its easy synthesis, quickly became the preferred method for peptide synthesis, but also brought about a revolution in peptide synthesis, and became an independent discipline--solid phase organic synthesis, solid-phase synthesis of peptide synthesis was invented to promote automation. World's first real peptide synthesis apparatus appeared in the early 1980 's.
liquid-phase synthesis

based on a single n-α-protected amino acids on repeatedly adding the amino component the synthesized step by step, usually starting from the synthesis of c-Terminal amino acid chains, connected by DCC of a single amino acid, mixed carbon anhydride, or N-carboxy anhydride method. Carbodiimide include connected with DCC connection Agent n-and c-protected amino acids. It is important, which reagent n-protected amino acids their carbon-based and c deterioration in the protection of the free amino groups of amino acids, forming peptide chain, while output N,N?/FONT>-dyaylcohercylurea by-products. However, this method of racemization due to its side effects, or in an alkaline form 5 (4H)-oxaylones and N-acylurea are affected. Fortunately, these side effects can be minimized, but cannot be completely eliminated. Method is to join a connection like HoSu or HoBT catalyst and, in addition, this method can also be used in the synthesis of n-protected amino acid active ester derivatives. In turn spontaneous and active esters produced by any other c-protected amino acids or peptides forming new peptides