Peptide synthesis instrument types

peptide synthesis instrument is used to instrument for peptide synthesis, peptide synthesis steps are tedious, time-consuming, several companies developed automated peptide synthesizer. General is divided into two class, a is for number less but synthesis volume big of production type application, typical representative is United States ABI company of ABI336 type more peptide synthesis instrument, its reactor turned way is different from Yu Qian two generation of more peptide synthesis instrument, that reactor above relative fixed, and below for circle 360 degrees fast rotating, led reactor in of solid liquid two-phase from bottom up for spiral movement, has been reached reactor of most above; addition a more peptide synthesis instrument for Yu drug development, and gene research, research sex application, Is typically represented by Germany Intavis AG company ResPep SL and MultiPep RS series, can provide both solid-phase synthesis and application of membrane synthesis and screening can help customers automatically maps complete high-throughput peptide synthesis at work.
peptide synthesis instrument classification of
Advent of peptide synthesis instrument contributed greatly to the development of peptide science. In turn, with the development of peptide science, scientists have also put forward higher requirements for synthesizer, which led to the development of the synthesizer. Currently more peptide synthesis instrument varieties range, from synthesis volume Shang points, can is divided into µg level of, mg level of, grams level of and kg level of; from function Shang points, can is divided into research type of, small try type of, in the try type of, general production type of and GMP production type of; from Automation degree Shang points, can is divided into automatic of, semi-automatic of and manual of; from channel Shang points, can is divided into single channel of and more channel of; from technology angle Shang points, can is divided into first generation of, second generation of, and third generation of; and so on.
first generation peptide synthesizer] first generation peptide synthesizer produced from the late 60 to early 70 's. B] represents the product is Beckman of Beckman company 990 Peptide Synthesizer[1] and Vega's of Vega's Biotechnologies company 296 Peptide Synthesizer. Now, the two companies were to give up the peptide synthesis instrument development and production, we can only be found in the academic literature in the early design theory and research. With the development and improvement of the production process, first generation peptide synthesizer has been withdrawn from the market. But prior to 1990 the number of peptide chemistry literature were experimental device developed to run on to, first generation peptide synthesis apparatus for the synthesis of instrument development and manufacturing have a significant meaning. Second generation peptide synthesizer] emergence of second generation peptide synthesizer in the 80 's of the last century. B] represents the product is Protein Technologies the company introduced the PS3 Peptide Synthesizer, as well as ACT peptide synthesizer of Advanced ChemTech company Model 90. Landmark is characterized by mild reaction synthesis of peptides, can generally be divided into both pure nitrogen bubbling and shake. PS3 design principle is to use nitrogen bubbling reaction to mixing the reactants, reactor is fixed on the synthesizer, nitrogen gas discharged from the reactor underneath by reactor to the upper, bubbles produced in the course of solid and liquid mixing. Such design of benefits is structure simple, cost low, but reaction relative mild: 1) sometimes more peptide-solid carrier in electrostatic role Xia will "hold mission", makes its cannot and liquid phase full mixed, in this situation Xia need adjustable high nitrogen of pressure to elimination electrostatic role; and in electrostatic role elimination Hou to put pressure immediately adjustable low, otherwise high of pressure will put more peptide-solid carrier "blow" to reactor liquid surface above. Because the peptide-solid phase carrier has a strong sense of adhering to the wall, once glued to the reactor above the liquid surface could no longer, or can no longer participate in the reaction. Apparently the first generation machine is not automatic as the pressure increases, this is causing the reaction, "dead end" an important reason. Reaction corner will reduce efficiency and purity of peptides in peptide synthesis, synthesis or even failure. 2) volatile nitrogen bubbling will make solution for a long time, reduced part of the peptide-solid phase carrier liquid surface sticking above the surface, or can no longer participate in the reaction. 3) nitrogen consumption, operating costs increased. ACT90 design principle is the reactor around the origin in the upright swing, or circular motion. ACT of peptide synthesis instrument also has the characteristic of reaction, that is, rotation angle and speed limits cannot fully achieve the coupling of amino acids, reaction often requires a long period of time. The third generation peptide synthesizer] third generation peptide synthesizer was born in the 90 's of the last century b]] representative products are United States b]Applied Biosystems company ABI 433 peptide synthesizer, c s Bio's CS336 is no dead peptide synthesis instrument and Peptide Scientific Inc company produces a full range of 180-270 of mechanical stirring peptide synthesizer ... ABI433 design principles are relatively fixed over the reactor, and below the circular 360 degree spin, driven reactor of solid-liquid two-phase spiral motion from the bottom up, until we reach the top of the reactor. In other words, the solution can be reached at any point inside the reactor, truly free corners. Because of the mixing rate of 1800 revolutions per minute speed, response fully. Because there were no dead angle agitation to ensure purity of peptide synthesis, ABI433-peptide synthesis apparatus (its exit peptide synthesizer market instruments the final paragraph) so far in the world, also has large proportions. Of course, the ABI products price is also high. Because a component using high frequency, can often damage the solenoid valve, and ABI 7 solenoid valve modular design, bad solenoid valves have to replace the whole module, which increases maintenance costs. CS336 design principle is the midpoint of the reactor as the Center and rotate 180 degrees up and down mixing, mixing speeds of up to 180rpm, while its superiority by nitrogen bubbling reaction, nitrogen blow as an optional response into response methods, peptide synthesis instrument in the field of scientific research of high interaction effects are fully reflected. Fourth generation more peptide synthesis instrument due to 180 degrees no corner of mixing mode get has increasingly more of customer of recognized, Peptide Scientific Inc company from 2002 began will 180 degrees upper and lower flip of mechanical promise adjustable speed mixing mode as its development of full products of logo sex features, from PSI200, and 300, and 400, and 500 to 600 full series more peptide synthesis instrument are used 180-270 degrees upper and lower flip of mechanical mixing mode, PSI uses CNC motor system, mixing speed stepless adjustable, mixing in physical strength and speed to maximize energy. Fifth-generation peptide synthesizer United States CEM company known for its protein organic reactions and equipment manufacturing, launched Liberty of microwave assisted peptide synthesis instrument . It uses microwave heating method, greatly improves the speed of response, the reaction rate increases several times before to peptide synthesizer, or even more than 10 times. Its inherent characteristics have been recognized by many research and development customers. Drug screening-peptide synthesis apparatus such as ACT introduced a 106-channel asynchronous automatic peptide synthesis instrument, 96 channels of AAPPTEC company has fully automated peptide synthesizer, PTI introduces 98-channel fully automated peptide synthesis instrument.